Designer Phages Kill Drug Resistant Infections

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Scientists used three phages (Muddy, BPs, and ZoeJ, left to right) to treat a teen’s life-threatening bacterial infection. R. M. Dedrick et al., Nature Medicine

Health Editor’s Note: Bacteriophages or phages are a virus that infects a bacteria and uses that bacteria to make more of itself (reproduce). Phages can have as few as four genes or as many as hundreds. Bacteriophages have more numbers than any other organism on earth. 

We are running into bacteria that are resistant to currently used antibiotics. In actuality phages have been used for decades as an alternative to antibiotics especially against multi-drug-resistant strains of bacteria….Carol 

 

Engineered phages treat drug-resistant infection

by Geri Piazza/National Institutes of Health

At a Glance

  • A mixture of engineered phages successfully treated a teenager’s life-threatening antibiotic-resistant infection.
  • The findings show that a library of phages could be used to select and engineer a personalized treatment for people with otherwise untreatable infections.

Bacterial infections that can’t be treated successfully with known antibiotics are a serious threat to health. Researchers have been searching for new ways to kill these resistant bacteria. Viruses called bacteriophages, or phages, are one method under study.

Phages prey on bacteria. They infect certain bacteria, replicate inside them, and burst out, killing the bacteria. Dr. Graham F. Hatfull’s research laboratory at the University of Pittsburgh has been building a collection of phages. Over many years, college students in a global science program have isolated more than 10,000 phages from nature.

Dr. Helen Spencer of the Great Ormond Street Hospital in London asked Dr. Hatfull to screen his library for phages that would be effective against the bacterium isolated from a gravely ill teenager. The teen had had a double lung transplant and was out of treatment options after a life-threatening infection had resisted treatment with antibiotics. The Mycobacterium abscessus isolated from the patient’s infection, dubbed GD01, was resistant to all nine antibiotics tested.

The study was funded in part by NIH’s National Institute of General Medical Sciences (NIGMS). Results were reported in Nature Medicine on May 8, 2019.


Testing revealed a phage named Muddy that killed GD01 efficiently. Two other phages, called ZoeJ and BPs, also showed potential. The research team engineered these two phages to be more efficient at killing GD01.

A mixture of the three phages proved effective against GD01 in the lab. Because this mixture showed potential, it was used to soak a gauze pad to lay over the teen’s infected chest wound. The next day, the teen received the mixture intravenously. Every 12 hours, the researchers gave one billion units of the phage mixture.

After one day of treatment, blood tests detected high levels of phages. The high levels suggested that the phages were infecting the bacteria, replicating inside, killing it, and being released into the bloodstream. The research team also detected phages in other body fluids.

After one month of receiving twice-a-day intravenous therapy, the teen began receiving phage-soaked gauze pads on the infected chest wound and numerous skin lesions every day. After 6 weeks of treatment, a PET scan showed that an enlarged lymph node near the liver had shrunk. After six months of treatment, the teen’s chest wound and skin lesions had improved. There were no significant side effects.

“The idea is to use bacteriophages as antibiotics—as something we could use to kill bacteria that cause infection,” Hatfull says. “We purified the phages, we gave them to the patient, and the patient got better.”

M. abscessus strains vary in which phages they are susceptible to, so finding the right phages for each patient will be a challenge. Future research may lead to a phage mixture that works more broadly.

 


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6 COMMENTS

  1. Polyvalent bacteriophages are still on sale in Russia, Georgia, and at least some of the ex-USSR countries.

  2. To continue the post below: I myself used them on journeys and to cure stomach infections with very good results (100% cures) on myself and my family and friends, while they were available and I kept some for a decade or more after they were no longer on sale, and they still worked.
    In 2003 I had an accident that left me with a broken leg, infected with a resistent bacterium. Each day the surgeons treating me would come and scrape away the flesh that had died in the previous 24hrs and dress my would with the latest mixture of antibiotics. I was looking at my leg being amputated.
    One day, I said to my wife: “I wish I had some bacteriophage!” To my utter astonishment she said: “Bacteriophage? That is on sale in my country!” A week or so later I had a bottle of it and I got the hospital to allow me to go home for the weekend. At home, we unwrapped the bandages and sprinkled some drops of it onto my wounds. The result was well beyond my expectations! In an hour or less the grey flesh turned pink and instead of dying a bit each day it grew a bit each day. That leg which was half gone is now as good as the other which was undamaged.
    The pharmaceutical industry is run by bandits and murderers.

  3. Polyvalent bacteriophages were sold over the counter, without prescription throughout the USSR and western Europe (I don’t know about the USA) fron the early 1920s until they disappeared from the Chemists’ shelves in Britain around 1962 and from France about 1970. Polyvalent meant they were a mixture of a great number of different phages so they were effective against a large number of bacteria. They have NEVER produced deleterious side effects.

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